The benefits of Phenoveu
A multiplexed immunofluorescence (IF) technique that allows the simultaneous detection of multiple protein biomarkers using the same tissue section.
Phenoveu protocol works with a wide range of samples including FFPE tissues.
Up to 60 iterations are possible based on the tissue type.
Our portfolio includes a wide range of conjugated antibodies.
We also offer conjugation of antibodies based on individual requirements.
How it works
The principle of this method is an amplified fluorescence signal using multiple fluorophores conjugated to a DNA barcode attached to a secondary antibody. This method allows researchers to use their own primary antibodies. Multiple fluorophore conjugation also enables the detection of low abundance proteins. Initially, primary antibodies bind to epitopes. Secondary DNA barcoded antibody then binds the primary antibody. These barcodes are later hybridized with complementary barcodes tagged with ten fluorophores, increasing the intensity and sensitivity of the stain while reducing background noise. For multiplexing, repeat the above steps for several rounds by introducing an intermediate stripping process.