Multiplex immunofluorescence (mIF) allows for the simultaneous detection of multiple tissue biomarkers for proteomics profiling. One of the promising approaches involves metal-conjugated antibodies, which can detect multiple protein markers in a single staining cycle. A pioneer in this field is the Hyperion Imaging Mass Cytometry (IMC) system from Standard BioTools. However, the overall manual staining process is very lengthy and laborious. Multiplexing using IMC workflow combines metal conjugated multiple antibodies (≥40) which enable the visualization of biomarkers using Hyperion IMC. Proteomics profiling using same tissue section provides in-depth details of molecular signaling and its surrounding microenvironment.
This blog describes our recent achieved milestone in automating the manual tissue staining process for metal-conjugated antibodies which is finally visualized through Hyperion IMC. Our state-of-the-art, fully automated staining system, NanoVIP®, efficiently reduced hands-on time and provided huge cost control in terms of reagent, antibody, and probe volume usage.
Hyperion Manual Staining:
The illustrations represent manual tissue staining workflow for IMC. Figure 1 represents the workflow for fresh frozen tissues. Figure 2 demonstrates the workflow for formalin-fixed, paraffin-embedded (FFPE) tissues.
NanoVIP® fully automated staining platform to automate Hyperion Manual Staining Steps
Omicsveu’s motto is to simplify all complicated manual slide-based staining processes with NanoVIP®. With its unique design, our instrument is a straight-forward plug & play system that gives reproducible and uniform staining results. All these features are evident with our recent most collaboration from Charles Perkins Centre, The University of Sydney where NanoVIP® successfully automated IMC staining protocols with unmatched staining quality and cutting all cost control factors.
Installation of NanoVIP® is remarkably simple with its plug & play concept and user-friendly software interface. In about 15 minutes time frame, users have already completed the set-up process for IMC staining protocol and NanoVIP® continues the staining process without further human intervention. With these, researches and technicians can focus their time on other activities. Overall, NanoVIP® allows researcher to accelerating research outcomes by performing more experiments with efficiency, increases productivity, empowering scientists to new findings and new research out comes.
One of the most notable benefits of NanoVIP® lies in its substantial cost savings in terms of time and reagent use. By utilizing a significantly reduced volume of reagents, NanoVIP® reduces reagent costs by up to 80%. With just 25 μl of reagents in a reaction, compared to the conventional requirement of ≥150 μl for Hyperion manual staining, NanoVIP® proves its value by delivering outstanding results while optimizing resource utilization.
Below representative images showcasing the exceptional precision and clarity of tonsil tissue staining achieved through the automated Hyperion protocol on NanoVIP®.
Advantages of Multiplexing and Automation of Manual Staining
The combination of the imaging system with the automation of manual staining steps has several advantages, including:
- Cost-effective: Reducing reagent costs and reducing the labor required for multiplex-proteomics makes it more cost-effective than conventional methods;
- Time-saving: Automating manual staining steps saves significant time and generates proteomics data for over 40 markers in a single staining cycle using a single tissue section; and
- Streamline Process: Using automated processes simplifies the process of proteomics profiling, making it more accessible to researchers.
The following table illustrates the key differences between Hyperion manual staining and the automation staining process on NanoVIP®.
The emergence of tissue multiplexing technology and the automation of manual staining steps has transformed the field of proteomics profiling. By combining technologies such as the Hyperion imaging system and NanoVIP®, researchers can perform proteomics profiling more efficiently, cost-effectively, and accurately, allowing for more comprehensive data analysis. These advancements have opened new avenues for research that will be exciting for future developments in spatial biology.
Explore the capabilities of NanoVIP® and how it can accelerate your spatial biology research.