In Situ Sequencing

  • Single-cell RNA sequencing has become the logical choice to overcome the cellular heterogeneity in tissue samples. However, spatial organization and localization of the transcript is necessary for a better understanding of pathophysiological conditions.
  • In situ sequencing allows us to sequence RNA transcripts directly in a fixed section or cells with sub-cellular level spatial information.
  • The method uses nucleic acid bases labeled with unique fluorescent dyes, padlock probes for target RNA’s, and the enzymes that catalyze the formation of circularized DNA for rolling circle amplification.
  • BioGenex Nano VIP®offers automated In Situ Sequencing and Fluorescence in-situ sequencing (FISSEQ).