Spatial Biology – Made Easy

Spatial biology is a field that studies the spatial distribution and interaction of biomolecules, viz., DNA, RNA, proteins, and metabolites in their native tissue context. Spatial Multiplex Multiomics is a field of spatial biology that aims to measure and map these multiple types of biomolecules (DNA, RNA, proteins, and metabolites) in native tissue context which can reveal the complex interactions and functions of cells and tissues in health and disease.

One of the leading providers of spatial multiplex multiomics products and services is Omicsveu, a company that offers a range of technologies, such as Phenoveu, RNAveu, miRNAveu, and In Situ Sequencing that enable the detection and visualization of multiple biomarkers in the same tissue section.  

Phenoveu: Allows simultaneous detection of multiple protein biomarkers using the same tissue section. 

RNAveu: Allows the detection of mRNA expression in sample with sub-micron level accuracy by using uniquely labelled fluorophore-tagged barcoded probes. 

miRNAveu: Allows the detection of target miRNA expression and can be combined with other technologies such as RNAveu and Phenoveu. 

In situ Sequencing technology: Examine the location of target mRNA sequences along with changes in single base pair mutations at the tissue and single cell level. 

RNAveu and Phenoveu

Omicsveu also provides a fully automated staining system, NanoVIP, that can process up to 64 In Situ spatial reactions or up to 96 sequencing-ready libraries in a ~2 days process. NanoVIP system offers a broad line of Spatial Multiplex Multiomics products consisting of a fully automated staining system, ready-to-use probes, multiplexing antibodies and visualization kits. Omicsveu’s technologies have been used to study various biological processes and diseases, such as tumor development and progression, immune response, and organ development. 

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Fully Automated Spatial Transcriptomics with RNAveu

Featured Highlights

  • Single copy gene visualization up to 40 plex
  • Fully automated using NanoVIP®
  • Ready-to-use reagents with fully optimized protocol

 

Introduction

Spatial transcriptomics is a revolutionary method that maps gene expression in tissue while preserving spatial information. It provides an unbiased map of RNA molecules throughout tissue sections, capturing the positional context of transcriptional activity within intact tissue. This technology is capable of assigning cell types (identified by the mRNA readouts) to their locations in the histological sections and can also be used to determine sub-cellular localization of mRNA molecules. In the vast landscape of genetics, where DNA orchestrates the symphony of life, RNAveu emerges as a beacon of precision & spatial transcriptomics research. Developed by Omicsveu, this cutting-edge technology unravels the intricate secrets encoded within RNA molecules. Let us delve into the fascinating world of RNAveu and its transformative impact.

 

What Is RNAveu?

RNAveu is a powerful molecular analysis platform that enables researchers to explore gene expression and chromosomal abnormalities at mRNA level with unparalleled precision. It can reveal cellular heterogeneity, spatial organization, and functional interactions in complex biological systems. This technology is particularly beneficial in fields such as developmental biology, cancer, immunology, and neuroscience. Here is how it works:

 

  1. Single Copy Gene Visualization:
    • RNAveu employs uniquely labeled fluorophore-tagged barcoded probes.
    • Enabled by cocktail of specific oligo probes and 10x signal amplification on each hybridization site.
    • These probes detect mRNA expression in samples as single copy gene.
  2. Targeted Insights:
    • Researchers can visualize single-copy genes using RNAveu.
    • With only two hybridization steps, RNAveu reveals the spatial distribution of RNA within cells.

Expression of PD1(orange), PDL1 (Green), TIM3 (Yellow) and LAG3(Red) in Non-small cell lung carcinoma (nucleus- Blue)

Expression of PD1(orange), PDL1 (Green), TIM3 (Yellow) and LAG3(Red) in Non-small cell lung carcinoma (nucleus- Blue)

 

Applications and Advancements in Spatial Transcriptomics:
  • RNAveu sheds light on the hidden world of tumor cells and its surrounding microenvironment and provide crucial insights in the fields of embryology, oncology, immunology, and histology.
  • By exploring spatial transcriptomics expression, researchers gain insights into tumor development and progression.
  • It allows for the detection of target mRNA expression, providing a comprehensive view of gene regulation.
  • Defining the spatial distribution of mRNA molecules allows for the experimentalist to uncover cellular heterogeneity in tissues, tumors, immune cells as well as determine the subcellular distribution of transcripts in various conditions.

 

Why RNAveu Matters

 

Spatial Biology Research and Precision Medicine:

  • RNAveu empowers personalized medicine by deciphering gene expression profiling.
  • Researchers can identify potential therapeutic targets and tailor treatments.

Unraveling Complexity:

  • RNAveu reveals the dynamic interplay of RNA molecules.
  • From non-coding RNAs to splicing variants, it uncovers hidden layers of genetic information.

Conclusion

As we journey deeper into the genomic landscape, RNAveu stands as a guiding light. Its ability to illuminate the intricate dance of RNA within cells opens new avenues for scientific discovery. Whether in cancer research, developmental biology, or disease mechanisms, RNAveu promises to revolutionize our understanding of genetics. This may lead to new strategies to prevent or treat infections, cancers, neurological or metabolic disorders, and a plethora of other conditions. With its unique capabilities, spatial transcriptomics is poised to make significant contributions to the field of gene expression analysis and beyond.

 

For more information, visit the RNAveu page and explore the possibilities of spatial biology with RNAveu and NanoVIP®.

Download RNAveu Brochure: http://bit.ly/RNAveu_Brochure

Automation of RNAveu with NanoVIP®: https://omicsveu.com/product/nanovip/

Contact us for more information on RNAveu: https://omicsveu.com/contact/

 

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Illuminating the hidden world of tumor cells: Exploring spatial transcriptomics expression with RNAveu

In the intricate domain of molecular biology, the advent of advanced techniques has made provision for scientists to hunt through deeper into the inner workings of cells and untangle the secrets hidden within. One such ground breaking technology that has emerged is sequential fluorescence in situ hybridization is a powerful molecular imaging technique used in the field of biology and transcriptomics to study the spatial organization and interactions of RNA molecules within individual cells and tissues. It is a type of RNA fluorescence in situ hybridization (RNA-FISH) that enables researchers to visualize the locations of multiple RNA species simultaneously with single-molecule sensitivity.

This blog post takes you on a journey through the fascinating world of sequential RNA-FISH (Omicsveu’s RNAveu) shedding light on its significance, applications, and potential to revolutionize our understanding of cellular processes.

Unveiling the Invisible: What is RNAveu?

RNAveu is a cutting-edge molecular imaging technique that allows scientists to peer into the heart of cells and tissues like never before. By combining the power of fluorescence microscopy and hybridization techniques, OmicsVeu’s RNAveu technology enable researchers to visualize and analyze multiple RNA molecules within the context of individual cells, tissues, and even whole organisms with exceptional precision. Unlike traditional techniques that primarily focus on protein expression, OmicsVeu’s sequential RNAveu offers a window into the world of gene expression by targeting specific RNA sequences (Figure 1).

Expression of PD1(ornage), PDL1 (Green), TIM3 (Yellow) and LAG3(Red) in Non-small cell lung carcinoma (nucleus- Blue)

Figure 1: Expression of PD1(ornage), PDL1 (Green), TIM3 (Yellow) and LAG3(Red) in Non-small cell lung carcinoma (nucleus- Blue)

How does RNAveu Work?

RNAveu process starts with series of innovative steps that combine principles of in situ hybridization and signal amplification. Here’s a simplified breakdown of the process:

  1. Probe Design: multiple short oligonucleotide probes, typically 25-30 bases long, are designed to be complementary to the target mRNA sequence. These probes are conjugated with unique barcode oligonucleotides typically 30-32 bases long.
  2. Complimentary barcode probe: A short oligonucleotide probes (25 bases long) are designed to be complimentary of unique barcode sequence. These probes are tagged with five fluorescent molecules each side of the oligo strand.
  3. Tissue Preparation: Tissues or cells of interest are carefully preserved and sectioned to maintain their structural integrity.
  4. Hybridization: The labeled RNA probes are applied to the tissue sections. These probes hybridize (bind) specifically to the target RNA sequences within the cells.
  5. Signal Amplification: Complimentary probe hybridization steps are performed to enhance the signal generated by the bound probes. This greatly increases the sensitivity of detection.
  6. Visualization: The labelled probes, now amplified, produce a visible signal that can be detected using fluorescence microscopy.

Multiple cycles of Revelation

OmicsVeu’s RNAveu builds colorful tissue morphology of RNA expression. It does this by performing multiple rounds of hybridization and imaging.

Here’s how it works:

  1. A set of probes specific to a particular RNA molecule is applied to the slide.
  2. The slide is imaged to capture the glowing spots where the probes have bound.
  3. The fluorescent markers are chemically removed or bleached, resetting the same slide for the next round.
  4. Steps 1 to 3 are repeated, each time using a new set of probes for different RNA molecules.
  5. The cumulative result is a detailed snapshot of the spatial distribution of various RNA molecules within a cell or tumor microenvironment.

Applications of RNAveu:

OmicsVeu’s RNAveu has proven to be a versatile tool with a wide range of applications across various fields of research:

  1. Cancer Research: Understanding the gene expression patterns in cancer cells can provide insights into disease progression and potential therapeutic targets.
  2. Neuroscience: It allows researchers to visualize gene expression in specific regions of the brain, aiding in the study of neurological disorders and brain development.
  3. Infectious Diseases: RNAveu has been instrumental in studying viral infections by enabling the visualization of viral RNA within infected cells.
  4. Pharmacology: Assessing the effects of drugs on gene expression patterns provides valuable information for drug development.

Advantages of RNAveu:

RNAveu offers several advantages over traditional techniques. It provides single-cell resolution, allowing researchers to analyze heterogeneity within a tissue sample. Additionally, it enables spatial analysis, offering insights into the localization of mRNA molecules within cells.

Looking ahead, this sequential RNA-FISH holds the potential to reshape our understanding of cellular processes and disease mechanisms. As the technique continues to evolve, it’s likely to become an even more indispensable tool in molecular and cellular biology research.

Conclusion

Sequential RNA-FISH (RNAveu) is a trailblazer in the realm of spatial transcriptomics, a field dedicated to understanding how RNA are expressed in their native context within tissues. It’s opening doors to even more advanced techniques that can simultaneously analyze thousands of genes across entire tissue sections, providing a comprehensive view of gene expression landscapes. Its ability to uncover the cellular profiling of mRNA within cells is transforming our understanding of tumor biology, development, and diseases. As RNAveu continues to evolve and inspire new technologies, the invisible world of cells is becoming increasingly visible, addressing a more accurate and awe-inspiring idea of personalized medicine.

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OmicsVeu Launches An Extensive Line Of Spatial Biology Products For Multiplex Multi-omics

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Infographic Transcript

 

OmicsVeu Launches an Extensive Line of Spatial Biology Products for Multiplex Multi-omics

OmicsVeu is pleased to announce the launch of a broad line of Multiplex Multi-Omics products for spatial biology consisting of a fully automated instrument NanoVIP® with optimized protocols, ready-to-use reagents for SuperPlex Phenotyping, Genotyping & Phenotyping kits, miRNA, mRNA & DNA FISH probes and kits, In Situ Sequencing system:

NanoVIP®: All-in-One fully automated slide staining system from microtome to slides ready for scanning & analysis using cell profiler, the likes. It is designed to automate any manual slide-based (FFPE, FF & CTC samples) assay protocol with intense, reproducible, consistent staining.  Read more.

SuperPlex Phenotyping: A multiplexed immunofluorescence (IF) technique that allows the simultaneous detection of multiple protein biomarkers using the same tissue section. Read more.

Genotyping & Phenotyping: Simple & reliable method for the simultaneous co-detection of protein, DNA miRNA & mRNA within the same tissue section. Read more.

miRFISH: A wide range of over 220 plus fluorophore-tagged miRNA probes enable the generation of multiplex miRNA profiling. These super-sensitive probes with high-melting temperatures are designed to detect low-abundant miRNAs. As miRNAs are tissue-specific, they help to diagnose cancer of unknown primary (CUP), poorly differentiated tumors and for the staging of cancer. Read more.

eFISH: This technology enables to detection of RNA & DNA with sub-micron level accuracy by using uniquely labeled fluorophore-tagged barcoded probes. Read more.

In Situ Sequencing: OmicsVeu introduces fully automated spatial transcriptomics sequencing technology with reagents, probes & a ready-to-use complete kit to elucidate transcriptomics profiling. This technology helps to empower CRISPR/CAS-mediated gene editing. Read more.

About OmicsVeu:

OmicsVeu is engaged in developing next-generation fully automated systems with optimized kits and ready-to-use reagents to accelerate the pace of Phenotyping, Genotyping, and Transcriptomics. All our products are easy-to-use and deliver reliable, consistent, and reproducible results with fast turnaround. Our systems are also open and work well with user-supplied reagents and have fully open intuitive software and provide ultimate detection sensitivity of a single nucleotide. We provide Premier Customer care and an unconditional performance guarantee.

OmicsVeu was founded to be one of the most innovative companies, accelerating multiplex multi-omics-systems for a better understanding of tumor micro-environment for precise tumor characterization, helping develop new therapeutics and innovative therapies for dramatically improved disease outcomes.

To accelerate the pace of Spatial Biology Research:

Apply for OmicsVeu Spatial Biology Grant Program

If you have any questions regarding the Grant Program, don’t hesitate to contact us here: grantinfo@omicsveu.com.

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